EZ DNA Methylation™ Kit (200 Rxns)

The EZ DNA Methylation Kit features a simplified procedure that streamlines bisulfite treatment of DNA. This kit is the original bisulfite conversion kit from Zymo Research. The EZ DNA Methylation Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Innovative desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including library preparation for Next-generation sequencing, PCR amplification, endonuclease digestion, sequencing, microarrays, etc. The EZ DNA Methylation Kit is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.

 

Applications	Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. This kit is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion	>99%
Elution Volume	≥10 µl
Equipment	Microcentrifuge and thermocycler with heated lid
Input	500 pg - 2 µg of DNA
Processing Time	12-16 hours
Recovery	>80%
Sample Source	Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.