2152777
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EZ-96 DNA Methylation™ Kit (Shallow-Well) (2 x 96 Rxns)

The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines...

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  • 2152777
  • ZYMO
  • D5003
The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput... mehr
The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc. Note: Catalog # 2152778  is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.

 

 

Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # 2152778  is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion >99%
Elution Volume ≥ 15 µl for Deep-well
≥ 30 µl for Shallow-well
Equipment Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input 500 pg - 2 µg of DNA
Processing Time 12-16 hours
Recovery >80%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

 

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