2152780
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EZ DNA Methylation-Gold™ Kit (200 Rxns)

The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation...

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497,00 € *

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Lieferzeit ca. 5 Tage

  • 2152780
  • ZYMO
  • D5006
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses... mehr
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

 

ApplicationsPurified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.
Conversion>99%
Elution Volume≥ 10 µl
EquipmentMicrocentrifuge and thermocycler with heated lid
Input500 pg - 2 µg of DNA.
Processing Time3 hours
Recovery>75%
Sample SourcePurified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
 
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