2152782
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EZ-96 DNA Methylation-Gold™ Kit (Deep-Well) (2 x 96 Rxns)

 The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion of unmethylated cytosines into uracil in less than 3 hours. Our optimized bisulfite chemistry...

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  • 2152782
  • ZYMO
  • D5008
 The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion... mehr
 The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion of unmethylated cytosines into uracil in less than 3 hours. Our optimized bisulfite chemistry combines heat denaturation and bisulfite conversion of input DNA to reduce incubation time and ensure conversion rates >99%. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

ApplicationsPurified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # 2152778 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion>99%
Elution Volume≥ 15 µl for Deep-well
≥ 30 µl for Shallow-well
EquipmentThermocycler with heated lid, swinging-bucket centrifuge with plate carriers
Input500 pg - 2 µg of DNA.
Processing Time3 hours
Recovery>75%
Sample SourcePurified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
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