2152804
Die Abbildung kann abweichen und Zubehör enthalten, das sich nicht im Lieferumfang dieses Artikels befindet.

EZ-96 DNA Methylation-Direct™ Kit (Shallow-Well) (2 x 96 Rxn

The EZ-96 DNA Methylation-Direct Kit is a 96-well bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the...

mehr ...

442,00 € *

zzgl. MwSt. zzgl. Versandkosten

Lieferzeit ca. 5 Tage

  • 2152804
  • ZYMO
  • D5022
The EZ-96 DNA Methylation-Direct Kit is a 96-well bisulfite conversion kit that features reliable... mehr
The EZ-96 DNA Methylation-Direct Kit is a 96-well bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this 96-well bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.

 

Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.
Conversion > 99.5%
Elution Volume Shallow well: > 30 µl
Deep-well: >15 µl
Equipment Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells.
Processing Time 4 hours
Recovery > 80%
Sample Source Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc.

 

Weiterführende Links zu "EZ-96 DNA Methylation-Direct™ Kit (Shallow-Well) (2 x 96 Rxn"
Verfügbare Downloads: