2152811
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EZ DNA Methylation-Lightning Kit (200 rxns.)

The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent...

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547,00 € *

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Lieferzeit ca. 5 Tage

  • 2152811
  • ZYMO
  • D5031
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than... mehr
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.

 

ApplicationsPurified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion> 99.5%
Elution Volume≥ 10 µl
EquipmentThermocycler with heated lid and microcentrifuge.
Input100 pg - 2 µg of DNA.
Processing Time1.5 hours
Recovery> 80%
Sample SourcePurified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
 
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