2152815
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EZ-96 DNA Methylation-Lightning Kit (Deep-Well) (2x96 rxns.)

The EZ-96 DNA Methylation-Lightning Kit is a rapid, high-throughput (96-well) bisulfite conversion kit for DNA methylation analysis. The streamlined workflow features ready-to-use Lightning...

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442,00 € *

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Lieferzeit ca. 5 Tage

  • 2152815
  • ZYMO
  • D5033
The EZ-96 DNA Methylation-Lightning Kit is a rapid, high-throughput (96-well) bisulfite... mehr
The EZ-96 DNA Methylation-Lightning Kit is a rapid, high-throughput (96-well) bisulfite conversion kit for DNA methylation analysis. The streamlined workflow features ready-to-use Lightning Conversion Reagent and a combined DNA denaturation and bisulfite conversion process. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. With the Deep-Well Kit (Cat. D5033), samples can be eluted in as little as 15 µl. High yield, converted DNA is ideal for PCR, array, and Next-Generation sequencing, etc.

 

ApplicationsPurified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion> 99.5%
Elution VolumeDeep-well: ≥ 15 µl
Shallow-well: ≥ 30 µl
EquipmentThermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input100 pg - 2 µg of DNA.
Processing Time1.5 hours
Recovery> 80%
Sample SourcePurified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
 
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