2152836
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EZ Nucleosomal DNA Prep Kit (20 Preps)

The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for the following: cell nuclei isolation, intact...

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139,00 € *

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Lieferzeit ca. 5 Tage

  • 2152836
  • ZYMO
  • D5220
The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of... mehr
The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for the following: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification. This kit includes two different enzymes for nucleosomal DNA preparation: Atlantis dsDNase and Micrococcal Nuclease. Enzymatic digestion yields very homogeneous populations of core nucleosomes and purification of the nucleosome-associated DNA is performed using Zymo Research’s proven spin column technology. The result is pure nucleosomal DNA ready for analysis in less than 45 minutes!

 

 

Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Processing Volume ≤106 cells
Purity Typical A260/A280 ≥ 1.8
Sample Source Nucleosome associated DNA isolation and purification from mammalian and yeast cells.
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Yield Up to 25 µg total DNA can be eluted into ≥ 25 µl. For DNA 75 bp - 10 kb the recovery is 70-90%. For DNA 11 kb - 23 kb the recovery is 50-70%.
Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Processing Volume ≤106 cells
Purity Typical A260/A280 ≥ 1.8
Sample Source Nucleosome associated DNA isolation and purification from mammalian and yeast cells.
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Yield Up to 25 µg total DNA can be eluted into ≥ 25 µl. For DNA 75 bp - 10 kb the recovery is 70-90%. For DNA 11 kb - 23 kb the recovery is 50-70%.
Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Processing Volume ≤106 cells
Purity Typical A260/A280 ≥ 1.8
Sample Source Nucleosome associated DNA isolation and purification from mammalian and yeast cells.
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Yield Up to 25 µg total DNA can be eluted into ≥ 25 µl. For DNA 75 bp - 10 kb the recovery is 70-90%. For DNA 11 kb - 23 kb the recovery is 50-70%.
Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Processing Volume ≤106 cells
Purity Typical A260/A280 ≥ 1.8
Sample Source Nucleosome associated DNA isolation and purification from mammalian and yeast cells.
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Yield Up to 25 µg total DNA can be eluted into ≥ 25 µl. For DNA 75 bp - 10 kb the recovery is 70-90%. For DNA 11 kb - 23 kb the recovery is 50-70%.
Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Processing Volume ≤106 cells
Purity Typical A260/A280 ≥ 1.8
Sample Source Nucleosome associated DNA isolation and purification from mammalian and yeast cells.
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Yield Up to 25 µg total DNA can be eluted into ≥ 25 µl. For DNA 75 bp - 10 kb the recovery is 70-90%. For DNA 11 kb - 23 kb the recovery is 50-70%.
 
Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Processing Volume ≤106 cells
Purity Typical A260/A280 ≥ 1.8
Sample Source Nucleosome associated DNA isolation and purification from mammalian and yeast cells.
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Yield Up to 25 µg total DNA can be eluted into ≥ 25 µl. For DNA 75 bp - 10 kb the recovery is 70-90%. For DNA 11 kb - 23 kb the recovery is 50-70%.

 

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