2152806
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EZ DNA Methylation-Startup™ Kit (50 Preps)

The EZ DNA Methylation-Startup Kit provides the necessary tools for complete bisulfite conversion of DNA for methylation analysis. This kit includes reagents that allow for bisulfite conversion of...

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434,00 € *

zzgl. MwSt. zzgl. Versandkosten

Lieferzeit ca. 5 Tage

  • 2152806
  • ZYMO
  • D5024
The EZ DNA Methylation-Startup Kit provides the necessary tools for complete bisulfite conversion... mehr
The EZ DNA Methylation-Startup Kit provides the necessary tools for complete bisulfite conversion of DNA for methylation analysis. This kit includes reagents that allow for bisulfite conversion of purified DNA and DNA directly from blood, cells, and fresh or FFPE tissues without the prerequisite for upstream DNA purification. A fully methylated Universal Methylated Human DNA Standard is provided with a special primer set for PCR to assess conversion efficiency. Finally, a unique ZymoTaq DNA Polymerase is included for robust amplification of bisulfite-treated DNA.

 

Applicable For Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.
Conversion > 99.5%
Elution Volume ≥ 10 µl
Equipment Thermocycler with heated lid and microcentrifuge.
Input Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells.
Processing Time 4 hours
Recovery > 80%
Sample Source Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc.

 

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