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Mix & Go! E.coli Transformation Buffer Set (up to 60 ml)

  The Mix & Go! E. coli Transformation Kit and Mix & Go! E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly...

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  • 2153091
  • ZYMO
  • T3002
  The Mix & Go! E. coli Transformation Kit and Mix & Go! E. coli Transformation Buffer Set... mehr

 

The Mix & Go! E. coli Transformation Kit and Mix & Go! E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Mix & Go! method completely eliminates the requirement for heat shocking and related procedures. Instead, Mix & Go! bacterial transformation can be performed by adding DNA to Mix & Go! competent cells and spreading onto a plate. Transformation efficiencies are typically on the order of 108-109 transformants/µg plasmid DNA with most E. coli strains. Uniquely formulated reagents make it easy to generate Mix & Go! competent cells from current E. coli strains that are available in the laboratory. Simply grow the E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready! The Mix & Go! E. coli Transformation Kit includes all buffers and ZymoBroth medium to generate 20 ml of Mix & Go! competent cells. The Mix & Go! E. coli Transformation Buffer Set includes all buffers that are required to generate 60 ml of Mix & Go! competent cells, and the medium (broth) is supplied by the user.

 

Compatible StrainsThe Kit is optimized to work with K12 and B derivative strains. Some commonly used strains include, TOP10, ccdB survival, XL1 Blue and Stbl3.
Processing Time≤ 45 minutes
Product StoragePlease store the Wash Buffer (2X), Competent Buffer (2X), and Dilution Buffer at 0-8°C. The ZymoBroth can be stored at room temperature.
Transformation Efficiency108-109 transformants per µg of plasmid DNA for most common lab strains.

 

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