2152759
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ZymoBIOMICS DNA Mini Kit (50 preps) (Lysis Tubes)

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The...

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274,00 € *

zzgl. MwSt. zzgl. Versandkosten

Lieferzeit ca. 5 Tage

  • 2152759
  • ZYMO
  • D4300
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a... mehr
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

 

Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 50 µl DNA Elution Buffer or DNase free water
Equipment Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing Volume Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2x109 bacterial, 2x108 yeast, or 2x107 mammalian cells)
Purity High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.
Sample Storage Eluted DNA should be stored at ≤ -20°C.
Size Range Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Type Total DNA
Yield Up to 25 µg total DNA can be eluted into ≥50 µl

 

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