2152765
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ZymoBIOMICS™ DNA Miniprep Kit (5 Preps)

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The...

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40,00 € *

zzgl. MwSt. zzgl. Versandkosten

Lieferzeit ca. 5 Tage

  • 2152765
  • ZYMO
  • D4300T
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a... mehr
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

 

Applicable ForAll sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume≥ 50 µl DNA Elution Buffer or DNase free water
EquipmentMicrocentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing VolumeFecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2x109 bacterial, 2x108 yeast, or 2x107 mammalian cells)
PurityHigh quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
Sample SourceBacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.
Sample StorageEluted DNA should be stored at ≤ -20°C.
Size RangeTypically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
TypeTotal DNA
YieldUp to 25 µg total DNA can be eluted into ≥50 µl
 
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