Quick-DNA/RNA FFPE Kit (50 Preps)

The Quick-DNA/RNA™ FFPE Kits provide a simple and reliable method for total nucleic acid isolation (DNA & RNA) from the same formalin-fixed, paraffin embedded (FFPE) tissue sample. The unique chemistries of the product have been optimized for maximum recovery of DNA and of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin™ column technology. The result is high-quality DNA & total RNA (including small RNAs 17-200 nt) that is DNA-free- and is ready for RT-PCR, hybridization, sequencing, etc.

 

Equipment	Microcentrifuge, vortex, and heat block/bath
Purity	DNA/RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: ≥1.8.
Sample Source	Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 mm2
Size Range	DNA and Total RNA ≥17 nt
Yield	50 µg DNA/RNA (binding capacity), ≥25 µl (elution volume)