Zymoprep™ Yeast Plasmid Miniprep I (100 Preps)

The Zymoprep Yeast Plasmid Miniprep I is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, and there is no need for glass beads or phenol. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard to isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.

 

Applicable For	Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications
Elution Volume	≥ 35 µl
Equipment	Microcentrifuge & Heat Block/Bath
Processing Volume	≤ 1.5 ml of Culture
Sample Source	Cell Culture (Colonies/Patches or Liquid Culture)
Size Range	No Size Limit
Yield	Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures