2152747
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Zyppy-96™ Plasmid MagPrep Kit (8 x 96 Preps)

 The Zyppy-96 Plasmid MagBead Miniprep is the fastest high-throughput (96-well), Pellet-Free plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit...

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997,00 € *

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  • 2152747
  • ZYMO
  • D4102
 The Zyppy-96 Plasmid MagBead Miniprep is the fastest high-throughput (96-well), Pellet-Free... mehr
 The Zyppy-96 Plasmid MagBead Miniprep is the fastest high-throughput (96-well), Pellet-Free plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization Buffer. Plasmid DNA is then purified using the featured magnetic bead based technology. This straightforward procedure is compatible for either automated or manual processing. Eluted plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection. An overview of the purification procedure is shown below.

Applicable ForLigation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume≥ 30 µl
EquipmentMagnetic Stand, Heating Element, or Liquid Handler
Processing Time60 min
PurityTypical Abs 260/280 ≥1.8.
Size RangeUp to 25 kb
YieldUp to 5 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli processed.
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