ZymoPURE II™ Plasmid Midiprep Kit (50 Preps)

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

Applicable For	Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.
Binding Capacity	400 µg
Culture Input	≤ 50 ml
Elution Volume	≥ 100 µl
Endotoxin Levels	≤ 0.025 EU/µg Plasmid DNA
Equipment	Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge
Format	Spin Column
Processing Time	20 min
Purity	Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
Size Range	Up to 200 kb
Yield	≤ 400 µg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)