2152751
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ZymoPURE II™ Plasmid Maxiprep Kit (20 Preps)

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade...

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382,00 € *

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Lieferzeit ca. 5 Tage

  • 2152751
  • ZYMO
  • D4203
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the... mehr
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

 

Applicable ForTransfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.
Binding Capacity1.2 mg
Culture Input≤ 150 ml
Elution Volume≥ 200 µl
Endotoxin Levels≤ 0.025 EU/µg Plasmid DNA
EquipmentMicrocentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge.
FormatSpin Column
Processing Time20 min
PurityTypical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
Recommended Sample Volume≤ 150 ml
Size RangeUp to 200 kb
Yield≤ 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)
 
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