2152756
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ZymoPURE™ Plasmid MiniPrep Kit (400 Preps)

Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA...

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545,50 € *

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Lieferzeit ca. 5 Tage

  • 2152756
  • ZYMO
  • D4211
Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin... mehr
Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

 

Applicable ForTransfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications.
Binding Capacity100 µg
Culture Input≤ 5 mL
Elution Volume25 µl
Endotoxin Levels≤ 1 EU/µg
EquipmentMicrocentrifuge and/or vacuum manifold
FormatSpin Column
Processing Time15 min
PurityTypical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
Recommended Sample Volume≤ 5.0 ml
Size RangeUp to ~200 kb
YieldUp to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized.
 
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