2153038
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Quick-RNA Fecal/Soil Microbe MicroPrep™ Kit (50 Preps)

The Quick-RNA Soil/Fecal Microbe Microprep is an innovative fecal and soil RNA extraction kit designed for the simple, reliable, and rapid isolation of total RNA including small RNAs (>17 nt) from...

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345,00 € *

zzgl. MwSt. zzgl. Versandkosten

Lieferzeit ca. 5 Tage

  • 2153038
  • ZYMO
  • R2040
The Quick-RNA Soil/Fecal Microbe Microprep is an innovative fecal and soil RNA extraction kit... mehr
The Quick-RNA Soil/Fecal Microbe Microprep is an innovative fecal and soil RNA extraction kit designed for the simple, reliable, and rapid isolation of total RNA including small RNAs (>17 nt) from various soil, sludge, sediment and/or fecal samples. The procedure of this fecal and soil RNA extraction kit successfully isolates RNA from tough-to-lyse bacteria, fungi, protozoa (protist), algae, etc. in soil, and host RNA from fecal samples. Samples are added to the ZR BashingBead Lysis Tube with an optimally designed S/F RNA Lysis Buffer where microbes are then lysed by bead beating to extract total RNA. The Zymo-Spin column technology included in this fecal and soil RNA extraction kit allows for quick filtration, genomic DNA removal from sample lysates, and isolation of the RNA. Zymo-Spin III-HRC Spin Filter separates RT-PCR inhibitors (e.g., humic acids, polyphenols, tannins) and the total RNA is concentrated using the Zymo-Spin IC Column with a minimum elution volume of ≥6 µl.

 

Equipment Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional)
Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Bacteria, fungi, protozoa, and algae in up to 250 mg soil, sludge or sediments, and bacteria, protist and/or host RNA from feces (mammalian, avian, etc.)
Size Range Total RNA ≥ 17 nt
Yield 10 µg RNA (binding capacity), ≥6 µl (elution volume)

 

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