His-Spin Protein Miniprep™ Kit (50 Preps)

The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 µl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.

 

Affinity Matrix	Nickel charged agarose
Binding Capacity	1 mg
Elution Method	Imidazole gradient
Elution Volume	100 - 200 µl (150 µl is optimal)
Principle of Technology	Based on a nickel-charged His-Affinity Gel (IMAC).
Processing Time	5 minutes
Product Storage	Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature.
Protein Purity	Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.
Required Equipment	Microcentrifuge
Sample Type	Cell lysates or other complex protein mixtures containing His-tagged proteins.