2152986
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His-Spin Protein Miniprep™ Kit (50 Preps)

The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as...

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302,00 € *

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Lieferzeit ca. 5 Tage

  • 2152986
  • ZYMO
  • P2002
The His-Spin Protein Miniprep provides a fast spin-column based purification technology for... mehr
The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 µl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.

 

Affinity MatrixNickel charged agarose
Binding Capacity1 mg
Elution MethodImidazole gradient
Elution Volume100 - 200 µl (150 µl is optimal)
Principle of TechnologyBased on a nickel-charged His-Affinity Gel (IMAC).
Processing Time5 minutes
Product StoragePlease store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature.
Protein PurityElectrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.
Required EquipmentMicrocentrifuge
Sample TypeCell lysates or other complex protein mixtures containing His-tagged proteins.
 
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