2152993
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Strep-Spin Protein Miniprep™ (50 preps)

Fast: Purify Strep-tagged proteins from cell lysates in less than 7 minutes. Simple: Prepare pure protein for small-scale studies using a spin-column. Convenient: No special instrumentation needed...

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302,00 € *

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  • 2152993
  • ZYMO
  • P2005
Fast: Purify Strep-tagged proteins from cell lysates in less than 7 minutes. Simple: Prepare pure... mehr
Fast: Purify Strep-tagged proteins from cell lysates in less than 7 minutes.

Simple: Prepare pure protein for small-scale studies using a spin-column.
Convenient: No special instrumentation needed other than a benchtop microcentrifuge.

Strep-Spin Protein Miniprep™ (10 preps)The Strep-Spin Protein Miniprep Kit™ provides a fast spin-column based purification technology for Strep-tagged proteins. Up to 450 µg of Strep-tagged protein can be purified in 7 minutes and eluted in Strep-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.

 

Affinity MatrixStrep-Tactin® XT Superflow® 50%
Binding Capacity100 µl of Strep-Tactin® XT Superflow® 50% suspension can bind 15 nmol biotin (approx. 450 µg of a 30 kDa Twin-Strep-tag® protein).
Elution MethodBiotin excess
Elution VolumeRecommended 3 x 200 µl
Principle of TechnologyThe Strep-Spin Protein Miniprep Kit is based on a novel StrepTactin® XT Superflow® resin which binds to Twin Strep-tag® with very high affinity. This tag contains two Strep-tag® II peptides connected via a Glycine/Serine linker sequence. Hence this kit is optimized for the purification of Twin-Step®-tagged proteins; however it will also work efficiently for proteins with single Strep-tags.
Processing Time7 minutes
Product StoragePlease store the Strep-Tactin® XT Superflow® 50% suspension, Strep-Wash Buffer, and Strep-Elution Bufer at 4°C. The other components can be stored at room temperature.
Protein PurityElectrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.
Required EquipmentMicrocentrifuge
Sample TypeCell lysates or other complex protein mixtures containing Strep-tagged proteins.


Required Equipment: Microcentrifuge
Sample Type: Cell lysates or other complex protein mixtures containing Strep-tagged proteins.

 
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