LightWave™ Plus Western Blot Lösung, 250ml

LightWave™ Plus is the best choice for mid-femtogram detection level. Extremely versatile, LightWave™ Plus enables the detection of the protein of interest when immunoblotting conditions are not yet optimized.

Its high sensitivity combined with a broad linear dynamic range allows an accurate quantification of both low and high abundance proteins in the same experiment. Furthermore, LightWave™ Plus extremely long signal duration results in superior reproducibility, ease of use, and less of a chance for creating artifacts.

Benchmarking data
LightWave™ Plus exhibits a mid-level performance for everyday experiments, offering a stable signal and a very good sensitivity. LightWave™ Plus can substitute, without changes in the protocol, the most common substrates, such as AmershamTM ECL Prime™ (GE Healthcare), Clarity™ (Biorad), Supersignal™ West Dura and West PICO PLUS (Thermo Scientific™). LightWave™ Plus provides an excellent performance in routine Western blotting applications, with higher signal intensity and sensitivity than several of its competitors, such as West PICO PLUS (Thermo Scientific™) and Clarity™ (Biorad) (Figure 1)

Features - Sensitivity and Precision
LightWave™ Plus produces a strong signal in the presence of a very low background level, resulting in a high signal-to-noise ratio and high sensitivity, comparable to AmershamTM ECL Prime™ (GE Healthcare) and SupersignalTM West Dura (Thermo Scientific™) and significantly better than Clarity™ (Bio-Rad) and Supersignal™ West PICO PLUS (Thermo Scientific™) (Figure 2). The high sensitivity, combined with its wide linear range, allows an excellent quantitation of low and high abundance proteins on the same blot, with a single exposure. LightWave™ Plus maximizes reproducibility, thus increasing the significance of experimental results

Signal duration
LightWave™ Plus provides an extremely extended signal duration when compared to most mid-level range ECL substrates. The HDAC-1 signal intensity variation over time was analyzed using LightWave™ Plus and its competitors (Figure 3).